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A colorimetric competitive displacement assay for the evaluation of catalytic peptides

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Abstract

An indicator displacement assay has been adapted to detect the diol products of the aldol reaction between 4-nitrobenzaldehyde and hydroxyacetone in crude reaction mixtures. This provides a rapid colorimetric method of detecting product formation and thus evaluating potential catalysts, which is demonstrated using multiple catalytic peptides.

Graphical abstract: A colorimetric competitive displacement assay for the evaluation of catalytic peptides

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Publication details

The article was received on 15 Aug 2017, accepted on 24 Oct 2017 and first published on 28 Nov 2017


Article type: Communication
DOI: 10.1039/C7OB02032E
Citation: Org. Biomol. Chem., 2017, Advance Article
  • Open access: Creative Commons BY-NC license
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    A colorimetric competitive displacement assay for the evaluation of catalytic peptides

    A. M. M. Gest, E. M. Aguiluz, M. T. Mays, X. Liu, E. K. Neidhart and L. S. Witus, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB02032E

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