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Issue 34, 2017
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pH-Responsive quencher-free molecular beacon systems containing 2′-deoxyuridine units labeled with fluorene derivatives

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Abstract

Three fluorescent nucleosides—UFL, UAF, and UDAF, containing fluorene, 2-aminofluorene, and 2-dimethylaminofluorene units, respectively, covalently attached to 2′-deoxyuridine—have been incorporated into the central positions of oligodeoxynucleotides (ODNs) to examine the effects of their flanking bases (FBs) and pH on the emission properties upon hybridization with fully matched and single-base-mismatched targets. The ODN containing UFL and cytosine-FBs in the pH range from 5.5 to 8.0 and the ODN containing UDAF and cytosine-FBs under slightly acidic conditions (pH 6.0–6.5) exhibited dramatic increases in fluorescence only upon duplex formation with their fully matched target DNAs.

Graphical abstract: pH-Responsive quencher-free molecular beacon systems containing 2′-deoxyuridine units labeled with fluorene derivatives

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Publication details

The article was received on 11 Jul 2017, accepted on 09 Aug 2017 and first published on 09 Aug 2017


Article type: Paper
DOI: 10.1039/C7OB01703K
Citation: Org. Biomol. Chem., 2017,15, 7165-7172
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    pH-Responsive quencher-free molecular beacon systems containing 2′-deoxyuridine units labeled with fluorene derivatives

    J. W. Lee, Y. Son, J. Y. Hwang, Y. Park and G. T. Hwang, Org. Biomol. Chem., 2017, 15, 7165
    DOI: 10.1039/C7OB01703K

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