Jump to main content
Jump to site search

Issue 32, 2017
Previous Article Next Article

Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

Author affiliations

Abstract

In this report, we show how a convenient on-resin copper-click functionalization of azido-functionalized peptide nucleic acids (PNAs) allows various PNA-based detection strategies. Firstly, a thiazole orange (TO) clicked PNA probe facilitates a binary readout when combined with F/Q labeled DNA, giving increased sensitivity for antisense detection. Secondly, our TO-PNA conjugate also allows single nucleotide polymorphism detection. Since antisense detection is also possible in the absence of the TO label, our sensing platform based on azido-D-ornithine containing PNA even allows for additional and more advanced functionalization and sensing strategies.

Graphical abstract: Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Jun 2017, accepted on 25 Jul 2017 and first published on 25 Jul 2017


Article type: Communication
DOI: 10.1039/C7OB01592E
Citation: Org. Biomol. Chem., 2017,15, 6710-6714
  •   Request permissions

    Facile functionalization of peptide nucleic acids (PNAs) for antisense and single nucleotide polymorphism detection

    D. Gahtory, M. Murtola, M. M. J. Smulders, T. Wennekes, H. Zuilhof, R. Strömberg and B. Albada, Org. Biomol. Chem., 2017, 15, 6710
    DOI: 10.1039/C7OB01592E

Search articles by author

Spotlight

Advertisements