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Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells

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Abstract

We developed a smart activity-based probe that detects the activity of asparaginyl endopeptidase (AEP) in live cells to monitor the dynamics of enzyme regulation. The newly designed probe generated a turn-on fluorescence signal in response to the activity of AEP in living cells without compromising the labelling efficiency or selectivity. Our probe closely reflected the enzyme activity in its native state, detecting subcellular AEP activity in colon cancer cells and neuronal cells.

Graphical abstract: Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells

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Publication details

The article was received on 16 Jun 2017, accepted on 12 Sep 2017 and first published on 12 Sep 2017


Article type: Communication
DOI: 10.1039/C7OB01467H
Citation: Org. Biomol. Chem., 2017, Advance Article
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    Development of a smart activity-based probe to detect subcellular activity of asparaginyl endopeptidase in living cells

    J. Hong, N. Choi, Y. La, H. Y. Nam, J. Seo and J. Lee, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB01467H

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