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2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo

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Abstract

Two 2-nitroimidazole-1,8-naphthalimide conjugates, 1 and 2, have been synthesised as fluorescence probes for the detection of reductive stress in HeLa cells. The 4-substituted derivative 1 was shown to act as a highly sensitive and selective substrate for nitroreductase where it exhibited a clear blue to green ratiometric fluorescence response visible to the naked eye. Moreover, biological studies demonstrated 1 could be activated in cellulo where the impact of reductive stress was easily monitored using confocal microscopy and flow cytommetry.

Graphical abstract: 2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo

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Publication details

The article was received on 09 Jun 2017, accepted on 10 Jul 2017 and first published on 10 Jul 2017


Article type: Communication
DOI: 10.1039/C7OB01406F
Citation: Org. Biomol. Chem., 2017, Advance Article
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    2-Nitroimidazole based fluorescent probes for nitroreductase; monitoring reductive stress in cellulo

    X. Ao, S. A. Bright, N. C. Taylor and R. B. P. Elmes, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB01406F

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