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Issue 20, 2017
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Chemical differentiation of three DMSP lyases from the marine Roseobacter group

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Abstract

Dimethylsulfoniopropionate (DMSP) catabolism of marine bacteria plays an important role in marine and global ecology. The genome of Ruegeria pomeroyi DSS-3, a model organism from the Roseobacter group, harbours no less than three genes for different DMSP lyases (DddW, DddP and DddQ) that catalyse the degradation of DMSP to dimethyl sulfide (DMS) and acrylate. Despite their apparent similar function these enzymes show no significant overall sequence identity. In this work DddQ and DddW from R. pomeroyi and the DddP homolog from Phaeobacter inhibens DSM 17395 were functionally characterised and their substrate scope was tested using several synthetic DMSP analogues. Comparative kinetic assays revealed differences in the conversion of DMSP and its analogues in terms of selectivity and overall velocity, giving additional insights into the molecular mechanisms of DMSP lyases and into their putatively different biological functions.

Graphical abstract: Chemical differentiation of three DMSP lyases from the marine Roseobacter group

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Publication details

The article was received on 12 Apr 2017, accepted on 03 May 2017 and first published on 09 May 2017


Article type: Paper
DOI: 10.1039/C7OB00913E
Citation: Org. Biomol. Chem., 2017,15, 4432-4439
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    Chemical differentiation of three DMSP lyases from the marine Roseobacter group

    I. Burkhardt, L. Lauterbach, N. L. Brock and J. S. Dickschat, Org. Biomol. Chem., 2017, 15, 4432
    DOI: 10.1039/C7OB00913E

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