Jump to main content
Jump to site search

Issue 21, 2017
Previous Article Next Article

Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification

Author affiliations

Abstract

Due to the lowered pKa of 4-fluorohistidine relative to histidine, peptides and proteins containing this amino acid are potentially endowed with novel properties. We report here the optimized synthesis of 4-fluorohistidine and show that it can efficiently replace histidine in in vitro translation reactions. Moreover, peptides containing 6×-fluorohistidine tags are able to be selectively captured and eluted from nickel resin in the presence of his-tagged protein mixtures.

Graphical abstract: Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification

Back to tab navigation

Supplementary files

Publication details

The article was received on 04 Apr 2017, accepted on 11 May 2017 and first published on 11 May 2017


Article type: Communication
DOI: 10.1039/C7OB00844A
Citation: Org. Biomol. Chem., 2017,15, 4536-4539
  •   Request permissions

    Genetic incorporation of 4-fluorohistidine into peptides enables selective affinity purification

    C. M. Ring, E. S. Iqbal, D. E. Hacker, M. C. T. Hartman and T. A. Cropp, Org. Biomol. Chem., 2017, 15, 4536
    DOI: 10.1039/C7OB00844A

Search articles by author

Spotlight

Advertisements