Jump to main content
Jump to site search


A novel off–on fluorescent probe for sensitive imaging of mitochondria-specific nitroreductase activity in living tumor cells

Author affiliations

Abstract

Sensitive and selective detection and imaging of nitroreductase (NTR) in cancer cells is of great importance for better understanding their biological functions. Since there are a few fluorescent probes concerning NTR activity specifically located in mitochondria, we developed a novel fluorescent benzoindocyanine probe (BICP) for mitochondrial NTR activity monitoring and imaging via extending a benzoindole moiety into a benzoindocyanine based fluorophore (BICF) with a strong intramolecular charge transfer (ICT) effect and incorporating 4-nitrobenzyl as a fluorescence-quenching and enzyme-responsive moiety. Live cell imaging of HeLa and A549 demonstrates that the developed BICP is able to realize sensitive and selective mitochondrial NTR activity probing with high-contrast “off–on” fluorescence. These findings implied the great potential of the developed probe for monitoring mitochondrial-specific NTR activities in living cells and related applications in cell biology.

Graphical abstract: A novel off–on fluorescent probe for sensitive imaging of mitochondria-specific nitroreductase activity in living tumor cells

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 29 Mar 2017, accepted on 27 Apr 2017 and first published on 27 Apr 2017


Article type: Paper
DOI: 10.1039/C7OB00781G
Citation: Org. Biomol. Chem., 2017, Advance Article
  •   Request permissions

    A novel off–on fluorescent probe for sensitive imaging of mitochondria-specific nitroreductase activity in living tumor cells

    B. Huang, W. Chen, Y. Kuang, W. Liu, X. Liu, L. Tang and J. Jiang, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB00781G

Search articles by author