Development of 19F-NMR chemical shift detection of DNA B-Z equilibrium using 19F-NMR
The various DNA conformational changes are correlation with biological event. In particular, DNA B-Z equilibrium was a high correlation with translation and transcription. In this study, we developed DNA probe containing 5-trifluoromethylcytidine or 5-trifluoromethylthymidine to detect DNA B-Z equilibrium using 19F-NMR. Its probe enable to detect the B-, Z-, and ss- DNA quantitatively based on 19F-NMR chemical shift change.