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Issue 17, 2017
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Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

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Abstract

Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, LacNAc-ITag, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.

Graphical abstract: Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

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Publication details

The article was received on 03 Mar 2017, accepted on 06 Apr 2017 and first published on 06 Apr 2017


Article type: Communication
DOI: 10.1039/C7OB00550D
Citation: Org. Biomol. Chem., 2017,15, 3575-3579
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    Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

    I. Sittel and M. C. Galan, Org. Biomol. Chem., 2017, 15, 3575
    DOI: 10.1039/C7OB00550D

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