Jump to main content
Jump to site search
PLANNED MAINTENANCE Close the message box

Scheduled maintenance upgrade on Thursday 4th of May 2017 from 8.00am to 9.00am (BST).

During this time our websites will be offline temporarily. If you have any questions please use the feedback button on this page. We apologise for any inconvenience this might cause and thank you for your patience.



Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

Author affiliations

Abstract

Imidazolium-labeled (ITag-) glycosides are used to harness the glycosyltransferase activity directly from human breast milk. The covalently attached ionic labels provide a bifunctional chemical handle that is used to monitor reaction progress by MS, as well as aid in product purification from complex mixtures. The technology is exemplified in the synthesis of biologically relevant oligosaccharide analogs, LacNAc-ITag, ITag-Lewisx and ITag-Lewisa, in a matter of days from human breast milk without having to isolate specific enzymes.

Graphical abstract: Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 03 Mar 2017, accepted on 06 Apr 2017 and first published on 06 Apr 2017


Article type: Communication
DOI: 10.1039/C7OB00550D
Citation: Org. Biomol. Chem., 2017, Advance Article
  •   Request permissions

    Imidazolium-labeled glycosides as probes to harness glycosyltransferase activity in human breast milk

    I. Sittel and M. C. Galan, Org. Biomol. Chem., 2017, Advance Article , DOI: 10.1039/C7OB00550D

Search articles by author