Issue 10, 2017

A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells

Abstract

Multi-functional supramolecular hydrogels have emerged as smart biomaterials for diverse biomedical applications. Here we report a multi-functional supramolecular hydrogel formed by the conjugate of the bioactive GRGDS peptide with biaryltetrazole that is the substrate of photo-click reaction. The hydrogel was used as a biocompatible matrix to encapsulate live cells for 3D culture. The presence of the RGD epitope in the hydrogelator enhanced the interaction of the nanofiber with integrin over-expressing cells, which resulted in the selective enhancement in the miRNA delivery into the encapsulated U87 cells. The intramolecular photo-click reaction of the biaryltetrazole moiety in the hydrogelator leads to a sensitive photo-response of the hydrogel, which allowed photo-degradation of the hydrogel for release of the encapsulated live cells for further bio-assay of the intracellular species.

Graphical abstract: A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells

Supplementary files

Article information

Article type
Paper
Submitted
06 Dec 2016
Accepted
07 Feb 2017
First published
07 Feb 2017

Org. Biomol. Chem., 2017,15, 2191-2198

A photo-degradable supramolecular hydrogel for selective delivery of microRNA into 3D-cultured cells

Z. Zhou, Q. Yi, T. Xia, W. Yin, A. A. Kadi, J. Li and Y. Zhang, Org. Biomol. Chem., 2017, 15, 2191 DOI: 10.1039/C6OB02667B

To request permission to reproduce material from this article, please go to the Copyright Clearance Center request page.

If you are an author contributing to an RSC publication, you do not need to request permission provided correct acknowledgement is given.

If you are the author of this article, you do not need to request permission to reproduce figures and diagrams provided correct acknowledgement is given. If you want to reproduce the whole article in a third-party publication (excluding your thesis/dissertation for which permission is not required) please go to the Copyright Clearance Center request page.

Read more about how to correctly acknowledge RSC content.

Social activity

Spotlight

Advertisements