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In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol

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Abstract

Understanding the physical properties of cholesterol–phospholipid systems is essential to gain a better knowledge of the function of each membrane constituent. We present a novel, simple and user-friendly setup that allows for the straightforward grazing incidence X-ray diffraction characterization of hydrated individual supported lipid bilayers. This configuration minimizes the scattering from the liquid and allows the detection of the extremely weak diffracted signal of the membrane, enabling the differentiation of the coexisting domains in DPPC:cholesterol single bilayers.

Graphical abstract: In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol

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Publication details

The article was received on 09 Oct 2017, accepted on 23 Nov 2017 and first published on 24 Nov 2017


Article type: Communication
DOI: 10.1039/C7NR07510C
Citation: Nanoscale, 2018, Advance Article
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    In-plane molecular organization of hydrated single lipid bilayers: DPPC:cholesterol

    B. Gumí-Audenis, L. Costa, L. Redondo-Morata, P. Milhiet, F. Sanz, R. Felici, M. I. Giannotti and F. Carlà, Nanoscale, 2018, Advance Article , DOI: 10.1039/C7NR07510C

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