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Polyethylenimine-coated Fe3O4 nanoparticles effectively quench fluorescent DNA, which can be developed as a novel platform for protein detection

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Abstract

We report a novel assembly of polyethyleneimine (PEI)-coated Fe3O4 nanoparticles (NPs) with single-stranded DNA (ssDNA), and the fluorescence of the dye labeled in the DNA is remarkably quenched. In the presence of a target protein, the protein–DNA aptamer mutual interaction releases the ssDNA from this assembly and hence restores the fluorescence. This feature could be adopted to develop an aptasensor for protein detection. As a proof-of-concept, for the first time, we have used this proposed sensing strategy to detect thrombin selectively and sensitively. Furthermore, simultaneous multiple detection of thrombin and lysozyme in a complex protein mixture has been proven to be possible.

Graphical abstract: Polyethylenimine-coated Fe3O4 nanoparticles effectively quench fluorescent DNA, which can be developed as a novel platform for protein detection

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Publication details

The article was received on 22 Sep 2017, accepted on 27 Oct 2017 and first published on 13 Nov 2017


Article type: Communication
DOI: 10.1039/C7NR07085C
Citation: Nanoscale, 2017, Advance Article
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    Polyethylenimine-coated Fe3O4 nanoparticles effectively quench fluorescent DNA, which can be developed as a novel platform for protein detection

    L. Ma, N. Sun, J. Zhang, C. Tu, X. Cao, D. Duan, A. Diao and S. Man, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR07085C

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