Jump to main content
Jump to site search


Enhanced and synergistic downregulation of oncogenic miRNAs by self-assembled branched DNA

Author affiliations

Abstract

miRNAs, a group of small non-coding RNA molecules, regulate the expression of many genes involved in various cellular processes. Acute evidence suggests that one miRNA can regulate many genes as its targets, while one gene can be targeted by many miRNAs that co-operatively regulate the gene. Thus, targeting a single miRNA is not sufficient enough to rescue the disease phenotype but it is also essential to target multiple miRNAs simultaneously. This inspired us to design a novel DNA nanostructure that can concurrently downregulate multiple oncomiRNAs. Here we designed a programmable antimiR branched DNA (antimiR-bDNA) nanostructure having antimiRNAs for selective binding to oncomiRNAs miRNA-27a, 96 and 182 which collectively downregulate FOXO1a expression. The antimiR-bDNAs show enhanced stability compared to naked antimiRNAs in serum and are able to knockdown these miRNAs with up to ∼50% greater repression as compared to antimiRNAs. This synergistic miRNA repression leads to the restoration of FOXO1a protein levels which in turn inhibit G1-S traversion in cancer cells. To the best of our knowledge, this is the first study harnessing the ability of bDNA structures to silence multiple miRNAs simultaneously.

Graphical abstract: Enhanced and synergistic downregulation of oncogenic miRNAs by self-assembled branched DNA

Back to tab navigation

Supplementary files

Publication details

The article was received on 05 Sep 2017, accepted on 16 Nov 2017 and first published on 21 Nov 2017


Article type: Paper
DOI: 10.1039/C7NR06601E
Citation: Nanoscale, 2017, Advance Article
  •   Request permissions

    Enhanced and synergistic downregulation of oncogenic miRNAs by self-assembled branched DNA

    S. Nahar, A. K. Nayak, A. Ghosh, U. Subudhi and S. Maiti, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR06601E

Search articles by author

Spotlight

Advertisements