Jump to main content
Jump to site search

Issue 44, 2017
Previous Article Next Article

A facile strategy for enzyme immobilization with highly stable hierarchically porous metal–organic frameworks

Author affiliations

Abstract

Metal–organic frameworks (MOFs) have drawn extensive research interest as candidates for enzyme immobilization owing to their tunable porosity, high surface area, and excellent chemical/thermal stability. Herein, we report a facile and universal strategy for enzyme immobilization using highly stable hierarchically porous metal–organic frameworks (HP-MOFs). The HP-MOFs were stable over a wide pH range (pH = 2–11 for HP-DUT-5) and met the catalysis conditions of most enzymes. The as-prepared hierarchical micro/mesoporous MOFs with mesoporous defects showed a superior adsorption capacity towards enzymes. The maximum adsorption capacity of HP-DUT-5 for glucose oxidase (GOx) and uricase was 208 mg g−1 and 225 mg g−1, respectively. Furthermore, we constructed two multi-enzyme biosensors for glucose and uric acid (UA) by immobilizing GOx and uricase with horseradish peroxidase (HRP) on HP-DUT-5, respectively. These sensors were efficiently applied in the colorimetric detection of glucose and UA and showed good sensitivity, selectivity, and recyclability.

Graphical abstract: A facile strategy for enzyme immobilization with highly stable hierarchically porous metal–organic frameworks

Back to tab navigation

Supplementary files

Publication details

The article was received on 14 Aug 2017, accepted on 21 Oct 2017 and first published on 23 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR06019J
Citation: Nanoscale, 2017,9, 17561-17570
  •   Request permissions

    A facile strategy for enzyme immobilization with highly stable hierarchically porous metal–organic frameworks

    X. Liu, W. Qi, Y. Wang, R. Su and Z. He, Nanoscale, 2017, 9, 17561
    DOI: 10.1039/C7NR06019J

Search articles by author

Spotlight

Advertisements