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Issue 43, 2017
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Yolk–shell magnetic mesoporous TiO2 microspheres with flowerlike NiO nanosheets for highly selective enrichment of phosphopeptides

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Abstract

In this work, we fabricated a yolk–shell magnetic composite that contains mesoporous TiO2 as the inner shell and flowerlike NiO as the outer shell (denoted as Fe3O4@H-TiO2@f-NiO) to reduce the limitations of single-component metal oxides in phosphopeptide enrichment. The NiO nanosheets play a synergistic role in phosphopeptide enrichment. And the unique flowerlike structure of NiO with sufficient space can facilitate the reversible insertion/extraction of peptides, which will have less impact on the enrichment process of the inner TiO2 shell. The yolk–shell structure and two types of porous nanostructures endowed this composite with a high surface area (156.58 m2 g−1) and a large pore volume (0.37 cm3 g−1). Owing to the high surface area and combined properties of TiO2 and NiO, the Fe3O4@H-TiO2@f-NiO microspheres showed a better performance for phosphopeptide enrichment than the same material without NiO nanosheets (Fe3O4@H-TiO2). According to the LC-MS/MS results, 972 unique phosphopeptides were identified from HeLa cell extracts with a high selectivity (91.9%) by Fe3O4@H-TiO2@f-NiO relative to 837 phosphopeptides (selectivity: 60.2%) by Fe3O4@H-TiO2. The results demonstrated that, compared with single-component metal oxides, composite metal oxides could enhance the selectivity and sensitivity for phosphopeptide enrichment.

Graphical abstract: Yolk–shell magnetic mesoporous TiO2 microspheres with flowerlike NiO nanosheets for highly selective enrichment of phosphopeptides

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Publication details

The article was received on 21 Jul 2017, accepted on 30 Sep 2017 and first published on 03 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR05330D
Citation: Nanoscale, 2017,9, 16764-16772
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    Yolk–shell magnetic mesoporous TiO2 microspheres with flowerlike NiO nanosheets for highly selective enrichment of phosphopeptides

    Y. Hong, C. Pu, H. Zhao, Q. Sheng, Q. Zhan and M. Lan, Nanoscale, 2017, 9, 16764
    DOI: 10.1039/C7NR05330D

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