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Multifunctional Gold Nanoparticle Layers for Controllable Capture and Release of Protein

Abstract

Protein modified functional surfaces have been applied extensively in the field of biomaterials and their medical applications. Regulation of the amount and activity of protein on the materials surface is always a challenge and a key research issue. A multifunctional micro/nano-composite based surface system for efficiently controllable capture and release of protein is proposed and studied in the present paper. This novel system contains: (1) gold nanoparticles (AuNP) co-modified with enzyme and poly(methacrylic acid) (PMAA), e.g., AuNP- pyrophosphatase (PPase)-PMAA, as nanostructured protein carriers; (2) gold nanoparticle layers (GNPLs) modified with poly(2-(dimethylamino)ethyl methacrylate) (PDMAEMA), i.e, GNPL-PDMAEMA, as micro/nano-structured support platform for surface bioactivity regulation. The capture-release of protein and the regulation of surface bioactivity in this composite surface system were investigated under different conditions. The results showed that the proposed system is capable of protein capture and release with simpler adjustment of the pH value from neutral pH to basic pH. When the system pH is stabilized at 7.0, the GNPL-PDMAEMA surface could adsorb plenty of AuNP-PPase-PMAA conjugates and maximal surface bioactivity occurred; but when the system pH is adjusted to 10.0, the GNPL-PDMAEMA surface could liberate almost all the AuNP-PPase-PMAA conjugates and thus surface bioactivity disappeared. Meanwhile, by cyclical variations between pH 7.0 and pH 10.0, this surface protein capture/release system could realize recycling and reuse of one certain protein multiple times, series of proteins acting sequentially in accordance with pre-designed procedures, and functional combination of multiple proteins. This recyclable multifunctional surface with the capability of protein capture/release has great potential in many applications, such as biomonitoring, biomolecule immobilization.

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Supplementary files

Publication details

The article was received on 19 Jul 2017, accepted on 10 Sep 2017 and first published on 11 Sep 2017


Article type: Paper
DOI: 10.1039/C7NR05276F
Citation: Nanoscale, 2017, Accepted Manuscript
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    Multifunctional Gold Nanoparticle Layers for Controllable Capture and Release of Protein

    Z. Li, F. Liu, Y. Yuan, J. Wu, H. Wang, L. Yuan and H. Chen, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR05276F

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