Jump to main content
Jump to site search


Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

Author affiliations

Abstract

On-the-spot visualization of biochemical responses of intact live cells is vital for a clear understanding of cell biology. The main obstacles for instant visualization of biochemical responses of living cells arise from the lack of a sophisticated detecting technique which can simultaneously provide chemical analysis tools and the biocompatible wet conditions. Here we introduce scanning transmission X-ray microscopy (STXM) combined with a liquid-enclosing graphene system (LGS), offering biocompatible conditions and improved X-ray absorption spectra to probe the chemical responses of live cells under wet conditions. This set-up enables us to probe a subtle change in absorption spectra depending on the oxidation state of a miniscule amount of oxygen in the functional groups present in each cell and its surroundings containing a minimal amount of liquid water. As an example of in situ biochemical responses of wet cells, chemical responses of a single Colo 205 cell are visualized and analyzed using X-ray absorption near the oxygen K-edge. This spectromicroscopic method using LGS can be applied to diverse biological samples under wet conditions for the analysis of their biochemical responses.

Graphical abstract: Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

Back to tab navigation

Supplementary files

Publication details

The article was received on 18 Jul 2017, accepted on 16 Oct 2017 and first published on 17 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR05223E
Citation: Nanoscale, 2017, Advance Article
  •   Request permissions

    Spectromicroscopic observation of a live single cell in a biocompatible liquid-enclosing graphene system

    S. U. Yu, H. Lee, W. J. Cho, C. Kim, M. C. Kang, H. Shin, N. Kim, S. K. Hahn and K. S. Kim, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR05223E

Search articles by author

Spotlight

Advertisements