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Biocompatible silver, gold and silver/gold alloy nanoparticles for enhanced cancer therapy: An in vitro and in vivo perspectives

Abstract

The aims of the nano oncology are to detect, target and treat cancer cells without any side effects. The present study describes the microbial synthesis of biocompatible silver (AgNPs), gold (AuNPs) and their alloy (Ag/AuNPs) nanoparticles for hepatoprotective activity against Diethylnitrosamine (DEN) induced liver cancer in Sprague Dawley (SD) rat models. Crystalline nature and physico-chemical features of nanoparticles were identified by fourier transform infra-red (FT-IR) spectroscopy, X-ray diffraction (XRD), X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and selected area electron diffraction (SAED) analysis. Based on the instrumental analysis, synthesised nanomaterials were found to be spherical in shape and have an average size in nano-regime. Nitrate reductase was characterized after partial purification of the culture filtrate via polyacrylamide gel electrophoresis and its molecular weight was determined as ~ 45 kDa. Furthermore, the IC50 values of AgNPs, AuNPs and Ag/AuNPs nanoparticles on HepG2 cells were determined as, 38.42 µg/ml, 43.25 µg/ml and 39.20 µg/ml respectively and antioxidant potential of the nanoparticles was also systematically analyzed. No-Observed-Adverse-Effect-Level (NOAEL) of AgNPs was determined as 2000 mg/kg of body weight (bw) from acute toxicity test. Similarly, NOAEL of AuNPs and Ag/AuNPs were calculated as 1000 mg/kg bw. Based on the in vivo studies, a significant tumour reduction (~ 45 to 65 %) was observed in the nanoparticles treated animals which was further confirmed by hematological, biochemical, TEM and histopathological analysis. Immunohistochemistry analysis confirmed the presence of BAX antibodies, upto immunoreactive (3+) level in treated animals. These results strongly suggest the potential anticancer activities of AgNPs, AuNPs and Ag/AuNPs against DEN induced liver cancer and could be a potential candidate for effective nano drug development

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Supplementary files

Publication details

The article was received on 10 Jul 2017, accepted on 11 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR04979J
Citation: Nanoscale, 2017, Accepted Manuscript
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    Biocompatible silver, gold and silver/gold alloy nanoparticles for enhanced cancer therapy: An in vitro and in vivo perspectives

    T. Shanmugasundaram, M. Radhakrishnan, V. Gopikrishnan , K. Kadirvelu and R. Balagurunathan, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04979J

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