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Microfluidics channel-coupled 3D quartz nanohole arrays for high capture and release efficiency of BT20 cancer cells

Abstract

Nanostructured materials, such as silicon nanowire, quartz nanostructures, and polymer-modified nanostructures, are a promising new class of materials for the capture and enumeration of very rare tumor cells, including circulating tumor cells (CTCs), to examine their biological characteristics in whole blood of cancer patients. These cells can then be applied towards transplantation, anti-tumor cell therapy, and cell-secreted protein studies. It is believed that 3-dimensional (3D) nanostructured substrates efficiently enhance cell capture yields due to the increased local contacts between the 3D nanostructures and extracellular extensions of the tumor cells. Recent studies have been performed with enhanced cell capture yields thanks to various nanostructured platforms; however, there remains an urgent need both to capture and release viable rare tumor cells for further molecular (i.e. protein) analysis and to develop patient-specific drugs. Here, we first demonstrate that our 3D quartz nanohole arrays (QNHA) tumor cell capture and release system allows us to not only selectively capture rare tumor cells, but also to release the cells with high capture and release rates. This system was developed with streptavidin (STR)-functionalized QNHA (STR-QNHA) with a microfluidics channel. Our system has ideal cell-separation yields of as high as 8591% and high release rates of >90% for BT20 cell line. We suggest that use of microfluidics channel technique coupled with STR-QNHA cell capture and release chip (STR-QNHA cell chip) would be a powerful and simple process to evaluate the capture, enumeration, and release of CTCs from patient whole blood for studying further cell therapy and tumor-cell-secreted molecules.

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Supplementary files

Publication details

The article was received on 09 Jul 2017, accepted on 01 Oct 2017 and first published on 02 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR04961G
Citation: Nanoscale, 2017, Accepted Manuscript
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    Microfluidics channel-coupled 3D quartz nanohole arrays for high capture and release efficiency of BT20 cancer cells

    J. Lim, Y. Yoon, W. Lee, J. Jeong, G. Kim, T. G. Kim and S. Lee, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04961G

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