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Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

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Abstract

In this contribution, we designed four types of immuno-magnetic nanoparticles for separation of different T cells (CD3+, CD4+, CD8+ and CD14+ T cells), and we established a new large-scale immuno-magnetic cell sorting procedure to achieve an enrichment of particular T cells using our designed auto-IMACS device. This device could achieve recyclable large-scale cell sorting, for which the throughput of the system reached ∼4000 mL and the maximum cell capacity was 4 × 1010. The collected cells were analyzed by flow cytometry and visual cytology data, and the effective selection rates of CD3+, CD4+, CD8+ and CD14+ T cells were 79.3%, 74.1%, 57.1% and 67.9%, respectively. The sorted CD8+ T cells still retained good cytotoxic activity against specific cells. In addition, the sorted T cells can also be further incubated in vitro and proliferated, and even could be infused back into patients for immunotherapy in the near future.

Graphical abstract: Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

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Publication details

The article was received on 07 Jul 2017, accepted on 22 Aug 2017 and first published on 23 Aug 2017


Article type: Paper
DOI: 10.1039/C7NR04914E
Citation: Nanoscale, 2017, Advance Article
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    Large-scale immuno-magnetic cell sorting of T cells based on a self-designed high-throughput system for potential clinical application

    Q. Zhang, T. Yin, R. Xu, W. Gao, H. Zhao, J. G. Shapter, K. Wang, Y. Shen, P. Huang, G. Gao, Y. Wu and D. Cui, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR04914E

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