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Surface-engineered quantum dots/electrospun nanofibers as a networked fluorescence aptasensing platform toward biomarker

Abstract

Membrane-based fluorescent sensing platform is a facile, point-of-care and promising technique in chemo/bio-analytical fields. However, the existing fluorescent sensing films for cancer biomarkers remain problematic: dissatisfactory sensitivity and selectivity, low utilization of probes encapsulated in films as well as tedious design of membranes structure. In this work, a novel fluorescent sensing platform by bio-grafting quantum dots (QDs) onto the surface of electrospun nanofibers (NFs) is fabricated. The aptamer integrated into the QDs/NFs can result in highly specific for recognizing and capturing biomarkers. A partial complementary DNA-attached gold nanoparticles (AuNPs) are employed to efficiently hybridize with the rest of aptamer to quench the fluorescence of QDs by nanometal surface energy transfer (NSET) between the both, which is constructed for prostate specific antigen (PSA) assay. Taking advantage of networked nanostructure of aptamer-QDs/NFs, the fluorescent film can detect PSA with high sensitivity and a detection limit of 0.46 pg/mL, which was further applied in clinical real serum samples. Coupling the surface grafted techniques to advanced network nanostructure of electrospun NFs, the proposed aptasensing platform can be easily extended to achieve sensitive and selective assay for other biomarkers.

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Publication details

The article was received on 04 Jul 2017, accepted on 25 Sep 2017 and first published on 26 Sep 2017


Article type: Paper
DOI: 10.1039/C7NR04817C
Citation: Nanoscale, 2017, Accepted Manuscript
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    Surface-engineered quantum dots/electrospun nanofibers as a networked fluorescence aptasensing platform toward biomarker

    T. Yang, P. Hou, L. L. Zheng, L. Zhan, P. F. Gao, Y. F. Li and C. Z. Huang, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04817C

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