Jump to main content
Jump to site search

Issue 41, 2017
Previous Article Next Article

Characterization of carboxylate nanoparticle adhesion with the fungal pathogen Candida albicans

Author affiliations

Abstract

Candida albicans is the lead fungal pathogen of nosocomial bloodstream infections worldwide and has mortality rates of 43%. Nanoparticles have been identified as a means to improve medical outcomes for Candida infections, enabling sample concentration, serving as contrast agents for in vivo imaging, and delivering therapeutics. However, little is known about how nanoparticles interact with the fungal cell wall. In this report we used laser scanning confocal microscopy to examine the interaction of fluorescent polystyrene nanoparticles of specific surface chemistry and diameter with C. albicans and mutant strains deficient in various C. albicans surface proteins. Carboxylate-functionalized nanoparticles adsorbed mainly to the hyphae of wild-type C. albicans. The dissociative binding constant of the nanoparticles was ∼150, ∼30 and ∼2.5 pM for 40, 100 nm and 200 nm diameter particles, respectively. A significant reduction in particle binding was observed with a Δals3 strain compared to wild-type strains, identifying the Als3 adhesin as the main mediator of this nanoparticle adhesion. In the absence of Als3, nanoparticles bound to germ tubes and yeast cells in a pattern resembling the localization of Als1, indicating Als1 also plays a role. Nanoparticle surface charge was shown to influence binding – positively charged amine-functionalized nanoparticles failed to bind to the hyphal cell wall. Binding of carboxylate-functionalized nanoparticles was observed in the presence of serum, though interactions were reduced. These observations show that Als3 and Als1 are important targets for nanoparticle-mediated diagnostics and therapeutics, and provide direction for optimal diameter and surface characteristics of nanoparticles that bind to the fungal cell wall.

Graphical abstract: Characterization of carboxylate nanoparticle adhesion with the fungal pathogen Candida albicans

Back to tab navigation

Supplementary files

Publication details

The article was received on 30 Jun 2017, accepted on 02 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR04724J
Citation: Nanoscale, 2017,9, 15911-15922
  •   Request permissions

    Characterization of carboxylate nanoparticle adhesion with the fungal pathogen Candida albicans

    A. Lyden, L. Lombardi, W. Sire, P. Li, J. C. Simpson, G. Butler and G. U. Lee, Nanoscale, 2017, 9, 15911
    DOI: 10.1039/C7NR04724J

Search articles by author

Spotlight

Advertisements