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A comparison of human and mouse protein corona profiles of functionalized SiO2 nanocarriers.

Abstract

Nanoparticles are a promising therapy against cancer as drug carriers given their versatile functionalization with polyethylene glycol and proteins that can be recognized by overexpressed receptors in tumor cells. However, it has been suggested that, in biological fluids, proteins cover nanoparticles, giving the proteins a biological identity that could be responsible for unexpected biological responses, the so-called protein corona. A relevant biological event usually ignored in protein-corona formation is the interspecies differences in protein binding, which can be involved in the discrepancies in preclinical studies and nanoparticle safety and efficiency. Hence, the aim of this study was to determine the differences between human and mouse plasma protein-corona profiles in a model of active therapy, silicon dioxide nanoparticles (SiO2 nanoparticles) functionalized with polyethylene glycol and transferrin. Functionalized SiO2 nanoparticles were obtained with 25 nm of primary particle size and pegylated with a cross-linker and a transferrin content of 50 μg/mg of nanoparticles. The proteomic analysis by nanoliquid chromatography tandem-mass spectrometry (nanoLC-MS/MS) showed interspecies differences. The most abundant proteins found in the human protein corona profile were immunoglobulins, actin cytoplasmic 1, hemoglobin subunit beta, serotransferrin, ficolin-3, complement C3, and apolipoprotein A-1. Meanwhile, mouse protein corona adsorbed the serine protease inhibitor A3K, serotransferrin, alpha-1-antitrypsin 1-2, hemoglobin subunit beta, and fibrinogen gamma and beta chains. These protein-corona profile differences of functionalized SiO2 nanoparticles indicate that biological responses found inside in vivo models could not be translated to clinical use and must be considered in the interpretation of preclinical trials in order to design more efficient and safer nanomedicines.

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Supplementary files

Publication details

The article was received on 29 Jun 2017, accepted on 19 Jul 2017 and first published on 25 Jul 2017


Article type: Paper
DOI: 10.1039/C7NR04685E
Citation: Nanoscale, 2017, Accepted Manuscript
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    A comparison of human and mouse protein corona profiles of functionalized SiO2 nanocarriers.

    A. Solorio-Rodríguez, V. Escamilla-Rivera, M. Uribe-Ramírez , A. Chagolla, R. Winkler, C. Garcia-Cuellar and A. De Vizcaya-Ruiz, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04685E

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