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Influence of Surface Coating on the Intracellular Behaviour of Gold Nanoparticles: A Fluorescence Correlation Spectroscopy Study

Abstract

In biomedical applications of nanoparticles (NP), the proper choice of surface chemistry is a crucial aspect in their design. The nature of the coating can heavily impact on the interaction of NPs with biomolecules, affect the state of aggregation, and ultimately determine their biological fate. As such, protein corona formation, and the aggregation behaviour of gold NPs (Au NPs) is studied here. Au NPs are prepared with four distinct surface functionalisations, namely: mercaptosuccinic acid (MSA), N-4-thiobutyroil glucosamine, HS-PEG5000 and HS-Alkyl-PEG600. Corona fomation, aggregation, and the intracellular behaviour of the Au NPs are then investigated by means of Fluorescence Correlation Spectroscopy (FCS) in cell culture media and in live cells. To evaluate the state of aggregation, and the formation of a protein corona, the Au NPs are incubated in cell media and the diffusion coefficient determined via FCS. The in vitro behaviour is compared with the level of aggregation of the NPs in cells. Diffusion times of the NPs are estimated at different positions in the cell after a one hour incubation period. It is found that the majority of MSA and Glucose- Au NPs are present inside the cell as slowly diffusing species with diffusion times (τD) greater than 6000 µs (hydrodynamic diameter >250 nm). PEGylated Au NPs adsorb a small amount of protein and manifest low agglomeration both in media and in living cells. In particular, the HS-Alkyl-PEG600 coating shows an excellent correlation between lower protein adsorption, 4-fold lower compared to the MSA coated NPs, and limited intracellular aggregation. In the case of single HS-Alkyl-PEG600 coated NPs it is found that typical intracellular τD range from 500-1500 μs,indicating that these particles display reduced aggregation in the intracellular environment.

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Publication details

The article was received on 27 Jun 2017, accepted on 01 Sep 2017 and first published on 01 Sep 2017


Article type: Paper
DOI: 10.1039/C7NR04640E
Citation: Nanoscale, 2017, Accepted Manuscript
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    Influence of Surface Coating on the Intracellular Behaviour of Gold Nanoparticles: A Fluorescence Correlation Spectroscopy Study

    A. Silvestri, D. di Silvio, I. Llarena, M. marelli, R. Murray, L. Lay, L. Polito and S. E. Moya, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04640E

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