Jump to main content
Jump to site search

Issue 6, 2018
Previous Article Next Article

A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient

Author affiliations

Abstract

Bronchoalveolar lavage (BAL) is a diagnostic procedure which samples the cellular and non-cellular components of the pulmonary epithelial surface. The inherent biological noise of BAL fluids inhibits their direct mineralogical analysis while currently available particle retrieval protocols are suspected to impose quantitative and qualitative bias on the studied particle load. This study presents a simple method for the near-lossless extraction of citrate-capped gold nanoparticles from human BAL fluids at sub-ppm levels which enables their quantitation and surface characterization. This procedure was modeled according to fundamental principles of particle sedimentation and liquid–liquid interdiffusion and was evaluated by a battery of analytical techniques. The extraction yield of gold nanoparticles ranged from 61 to 86%, with a quantitation limit at 0.5 μg ml−1, as measured by inductively-coupled optical emission spectroscopy. Dynamic light scattering could resolve the hydrodynamic size distribution of extracted particles which returned significantly different photon count rates at various concentrations. Their shape and primary size were easily observable by electron microscopy while atomic force microscopy, Auger electron spectroscopy and X-ray photoelectron spectroscopy could respectively probe the particles’ biomolecular corona, detect surface-adsorbed S- and N- species, and identify carbon-based covalent bonds.

Graphical abstract: A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient

Back to tab navigation

Supplementary files

Publication details

The article was received on 21 Jun 2017, accepted on 28 Dec 2017 and first published on 28 Dec 2017


Article type: Paper
DOI: 10.1039/C7NR04484D
Citation: Nanoscale, 2018,10, 2955-2969
  •   Request permissions

    A method for the quantitative extraction of gold nanoparticles from human bronchoalveolar lavage fluids through a glycerol gradient

    D. Bitounis, V. Barnier, C. Guibert, J. Pourchez, V. Forest, D. Boudard, J. Hochepied, P. Chelle, J. Vergnon and M. Cottier, Nanoscale, 2018, 10, 2955
    DOI: 10.1039/C7NR04484D

Search articles by author

Spotlight

Advertisements