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Attomolar Detection of Extracellular MicroRNAs Released from Living Prostate Cancer Cells by Plasmonic Nanowire Interstice Sensor

Abstract

Prostate cancer (PC) is the second leading cause of cancer death for men worldwide. Serum prostate-specific antigen level test has been widely used to screen for PC. This method, however, exhibits a high false-positive rate, leading to over-diagnosis and over-treatment of PC patients. Extracellular microRNAs (miRNAs) recently provided valuable information including the site and status of the cancers and thus emerged as new biomarkers for several cancers. Among them miR141 and miR375 are the most pronounced biomarkers for the diagnosis of high-risk PC. Herein, we report an attomolar detection of miR141 and miR375 released from living PC cells by using a plasmonic nanowire interstice (PNI) sensor. This sensor showed a very low detection limit of 100 aM as well as a wide dynamic range from 100 aM to 100 pM for all target miRNAs. In addition, the PNI sensor could discriminate perfectly the diverse single-base mismatches in the miRNAs. More importantly, the PNI sensor successfully detected the extracellular miR141 and miR375 released from living PC cell lines (LNCaP and PC-3), proving the diagnostic capability of the sensor for PC. We anticipate that the present PNI sensor can hold great promise for the precise diagnosis and prognosis of various cancer patients as well as PC patients.

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Publication details

The article was received on 19 Jun 2017, accepted on 07 Oct 2017 and first published on 10 Oct 2017


Article type: Paper
DOI: 10.1039/C7NR04386D
Citation: Nanoscale, 2017, Accepted Manuscript
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    Attomolar Detection of Extracellular MicroRNAs Released from Living Prostate Cancer Cells by Plasmonic Nanowire Interstice Sensor

    S. Yang, H. Kim, K. J. Lee, S. G. Hwang, E. Lim, J. Jung, T. J. Lee, H. Park, T. Kang and B. Kim, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR04386D

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