Jump to main content
Jump to site search

Issue 37, 2017
Previous Article Next Article

Mammalian cell defence mechanisms against the cytotoxicity of NaYF4:(Er,Yb,Gd) nanoparticles

Author affiliations

Abstract

Water-soluble upconversion nanoparticles (UCNPs), based on polyvinylpyrrolidone (PVP)-coated NaYF4:Er3+,Yb3+,Gd3+, with various concentrations of Gd3+ ions and relatively high upconversion efficiencies, were synthesized. The internalization and cytotoxicity of the thus obtained UCNPs were evaluated in three cell lines (HeLa, HEK293 and astrocytes). No cytotoxicity was observed even at concentrations of UCNPs up to 50 μg ml−1. The fate of the UCNPs within the cells was studied by examining their upconversion emission spectra with confocal microscopy and confirming these observations with transmission electron microscopy. It was found that the cellular uptake of the UCNPs occurred primarily by clathrin-mediated endocytosis, whereas they were secreted from the cells via lysosomal exocytosis. The results of this study, focused on the mechanisms of the cellular uptake, localization and secretion of UCNPs, demonstrate, for the first time, the co-localization of UCNPs within discrete cell organelles.

Graphical abstract: Mammalian cell defence mechanisms against the cytotoxicity of NaYF4:(Er,Yb,Gd) nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 24 May 2017, accepted on 17 Aug 2017 and first published on 18 Aug 2017


Article type: Paper
DOI: 10.1039/C7NR03705H
Citation: Nanoscale, 2017,9, 14259-14271
  •   Request permissions

    Mammalian cell defence mechanisms against the cytotoxicity of NaYF4:(Er,Yb,Gd) nanoparticles

    B. Sikora, P. Kowalik, J. Mikulski, K. Fronc, I. Kamińska, M. Szewczyk, A. Konopka, K. Zajdel, R. Minikayev, K. Sobczak, W. Zaleszczyk, A. Borodziuk, J. Rybusiński, J. Szczytko, A. Sienkiewicz, T. Wojciechowski, P. Stępień, M. Frontczak-Baniewicz, M. Łapiński, G. Wilczyński, W. Paszkowicz, A. Twardowski and D. Elbaum, Nanoscale, 2017, 9, 14259
    DOI: 10.1039/C7NR03705H

Search articles by author

Spotlight

Advertisements