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Issue 37, 2017
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Synthesis of a multi-functional DNA nanosphere barcode system for direct cell detection

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Abstract

Nucleic acid-based technologies have been applied to numerous biomedical applications. As a novel material for target detection, DNA has been used to construct a barcode system with a range of structures. This paper reports multi-functionalized DNA nanospheres (DNANSs) by rolling circle amplification (RCA) with several functionalized nucleotides. DNANSs with a barcode system were designed to exhibit fluorescence for coding enhanced signals and contain biotin for more functionalities, including targeting through the biotin–streptavidin (biotin–STA) interaction. Functionalized deoxynucleotide triphosphates (dNTPs) were mixed in the RCA process and functional moieties can be expressed on the DNANSs. The anti-epidermal growth factor receptor antibodies (anti-EGFR Abs) can be conjugated on DNANSs for targeting cancer cells specifically. As a proof of concept, the potential of the multi-functional DNANS barcode was demonstrated by direct cell detection as a simple detection method. The DNANS barcode provides a new route for the simple and rapid selective recognition of cancer cells.

Graphical abstract: Synthesis of a multi-functional DNA nanosphere barcode system for direct cell detection

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Publication details

The article was received on 21 May 2017, accepted on 17 Aug 2017 and first published on 21 Aug 2017


Article type: Paper
DOI: 10.1039/C7NR03615A
Citation: Nanoscale, 2017,9, 14094-14102
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    Synthesis of a multi-functional DNA nanosphere barcode system for direct cell detection

    S. Han, J. S. Lee and J. B. Lee, Nanoscale, 2017, 9, 14094
    DOI: 10.1039/C7NR03615A

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