Jump to main content
Jump to site search


Site-Specific Conjugation of Antibody on Gold Nanoparticle Surface for One-Step Diagnosis of Prostate Specific Antigen with Dynamic Light Scattering

Abstract

Small dimensions of gold nanoparticles (AuNPs) necessitate antibodies to be immobilized in an oriented fashion in order to conserve their antigen binding activity for proper function. In this study, we used the previously described UV-NBS method to site-specifically incorporate thioctic acid (TA) functionality into antibodies at the conserved nucleotide-binding site (NBS). Modified antibodies were immobilized on AuNP surface in an oriented manner utilizing the newly incorporated TA functionality while maintaining antibody structure and activity. The resulting antibody functionalized AuNPs via the UV-NBS method demonstrated significantly enhanced antigen detection capabilities and improved antigen detection sensitivity with high level of selectivity when compared to other commonly used AuNP functionalization methods. Our results demonstrate that the limit of detection (LOD) for AuNPs functionalized via the UV-NBS method was 55 pM PSA, which is 40, 851, and 5873-fold improved over the other immobilization methods: EDC-NHS, thiol reduction, and ionic interaction, respectively. Consequently, the UV-NBS method provides a universal, site-specific functionalization method that generates highly sensitive and more stable antibody functionalized AuNPs that is amenable to any available detection and treatment assay with potential significant implications.

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 01 May 2017, accepted on 17 May 2017 and first published on 18 May 2017


Article type: Paper
DOI: 10.1039/C7NR03096G
Citation: Nanoscale, 2017, Accepted Manuscript
  •   Request permissions

    Site-Specific Conjugation of Antibody on Gold Nanoparticle Surface for One-Step Diagnosis of Prostate Specific Antigen with Dynamic Light Scattering

    N. Mustafaoglu, T. Kiziltepe and B. Bilgicer, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR03096G

Search articles by author