Jump to main content
Jump to site search

Issue 25, 2017
Previous Article Next Article

Gold-loaded nanoporous iron oxide nanocubes: a novel dispersible capture agent for tumor-associated autoantibody analysis in serum

Author affiliations

Abstract

Autoantibodies are produced against tumor associated antigens (TAAs) long before the appearance of any symptoms and thus can serve as promising, non-invasive biomarkers for early diagnosis of cancer. Current conventional methods for autoantibody detection are highly invasive and mostly provide diagnosis in the later stages of cancer. Herein we report a new electrochemical method for early detection of p53 autoantibodies against colon cancer using a strategy that combines the strength of gold-loaded nanoporous iron oxide nanocube (Au@NPFe2O3NC)-based capture and purification while incorporating the inherent simplicity, inexpensive, and portable nature of the electrochemical and naked-eye colorimetric readouts. After the functionalisation of Au@NPFe2O3NC with p53 antigens, our method utilises a two-step strategy that involves (i) magnetic capture and isolation of autoantibodies using p53/Au@NPFe2O3NC as ‘dispersible nanocapture agents’ in serum samples and (ii) subsequent detection of autoantibodies through a peroxidase-catalyzed reaction on a commercially available disposable screen-printed electrode or naked-eye detection in an Eppendorf tube. This method has demonstrated a good sensitivity (LOD = 0.02 U mL−1) and reproducibility (relative standard deviation, %RSD = <5%, for n = 3) for detecting p53 autoantibodies in serum and has also been successfully applied to analyse a small cohort of clinical samples obtained from colorectal cancer. We believe that the highly inexpensive, rapid, sensitive, and specific nature of our assay could potentially aid in the development of an early diagnostic tool for cancer and related diseases.

Graphical abstract: Gold-loaded nanoporous iron oxide nanocubes: a novel dispersible capture agent for tumor-associated autoantibody analysis in serum

Back to tab navigation

Supplementary files

Publication details

The article was received on 27 Apr 2017, accepted on 07 Jun 2017 and first published on 09 Jun 2017


Article type: Paper
DOI: 10.1039/C7NR03006A
Citation: Nanoscale, 2017,9, 8805-8814
  •   Request permissions

    Gold-loaded nanoporous iron oxide nanocubes: a novel dispersible capture agent for tumor-associated autoantibody analysis in serum

    S. Yadav, M. K. Masud, Md. N. Islam, V. Gopalan, A. K. Lam, S. Tanaka, N. Nguyen, Md. S. A. Hossain, C. Li, Md. Y. Yamauchi and M. J. A. Shiddiky, Nanoscale, 2017, 9, 8805
    DOI: 10.1039/C7NR03006A

Search articles by author

Spotlight

Advertisements