Jump to main content
Jump to site search


Influence of Microenvironment Topography and Stiffness on Mechanics and Motility of Normal and Cancer Renal Cells

Abstract

Tumor microenvironment highly influences cancer cell modes and dynamics, above all during invasive and metastatic processes. When aiming to study cancer cell behavior in vitro, the use of conventional cell culture systems, such as Petri dishes, fails in recapitulating mechanical and topographical properties of the natural extracellular matrix (ECM). Here the versatility of stiffness-tunable hydrogels and the efficacy of replica molding technique with silicone polymers are exploited, aiming to study cancer and normal cell behavior with platforms able to capture the heterogeneities of the natural in vivo context. We compared mechanical properties of normal and cancer renal cells on different stiffness gels (with Young moduli of 3, 17 and 31 kPa) by using atomic force microscopy (AFM) and investigated cell indentation phenomena on compliant gels with confocal microscopy. Moreover, we studied cell mechanics, morphology and migration on isotropic linear structures, spaced at 1.5 µm, aiming to mimic the aligned fiber bundles typically observed at the tumor borders. By using this approach we could highlight differences on the way healthy and cancer renal cells react to changes in their microenvironment. Our results may potentially pave the way to unravel complex processes involved in cancer progression, especially in tissue invasion and migration during metastasis formation.

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Apr 2017, accepted on 04 Jul 2017 and first published on 06 Jul 2017


Article type: Paper
DOI: 10.1039/C7NR02940C
Citation: Nanoscale, 2017, Accepted Manuscript
  •   Request permissions

    Influence of Microenvironment Topography and Stiffness on Mechanics and Motility of Normal and Cancer Renal Cells

    C. Rianna and M. Radmacher, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR02940C

Search articles by author

Spotlight

Advertisements