Jump to main content
Jump to site search

Issue 30, 2017
Previous Article Next Article

Self-assembling covalent organic framework functionalized magnetic graphene hydrophilic biocomposites as an ultrasensitive matrix for N-linked glycopeptide recognition

Author affiliations

Abstract

The development of additional functions and applications of covalent organic framework (COF)-derived materials still remains highly desired. In our work, a novel COF-functionalized magnetic graphene biocomposite (MagG@COF-5) was first developed as an ultrasensitive hydrophilic matrix via a facile self-assembly method for efficiently recognizing N-linked glycopeptides. By integrating the characteristics of the magnetic graphene and COF-5 layer, the MagG@COF-5 owns features of an outstanding magnetic response, a high specific area, strong hydrophilic properties and a unique size-exclusion effect. Accordingly, the MagG@COF-5 biocomposite showed excellent performance in N-linked glycopeptide analysis with a low detection limit (0.5 fmol μL−1), an excellent size-exclusion effect (HRP digests/BSA, 1 : 600), good recyclability and reusability. More excitingly, the practical applicability of the biocomposite was evaluated by treatment with human serum (1 μL), in which 232 N-linked glycopeptides from 85 glycoproteins were detected. All the results demonstrate that the as-synthesized MagG@COF-5 biocomposite has huge potential for use in glycoproteome and clinical diagnosis fields. It will also open up new phases for application of COF-based materials.

Graphical abstract: Self-assembling covalent organic framework functionalized magnetic graphene hydrophilic biocomposites as an ultrasensitive matrix for N-linked glycopeptide recognition

Back to tab navigation

Supplementary files

Publication details

The article was received on 25 Apr 2017, accepted on 25 Jun 2017 and first published on 26 Jun 2017


Article type: Paper
DOI: 10.1039/C7NR02932B
Citation: Nanoscale, 2017,9, 10750-10756
  •   Request permissions

    Self-assembling covalent organic framework functionalized magnetic graphene hydrophilic biocomposites as an ultrasensitive matrix for N-linked glycopeptide recognition

    J. Wang, J. Li, M. Gao and X. Zhang, Nanoscale, 2017, 9, 10750
    DOI: 10.1039/C7NR02932B

Search articles by author

Spotlight

Advertisements