Jump to main content
Jump to site search


SDS-Assisted Protein Transport Through Solid-State Nanopores

Abstract

Using nanopores for the single-molecule sequencing of proteins – similar to nanopore-based sequencing of DNA – faces multiple challenges, including unfolding of the complex tertiary structure of the proteins and enforcing their unidirectional translocation through nanopores. Here, we combine molecular dynamics (MD) simulations with single-molecule experiments to investigate the utility of SDS (Sodium Dodecyl Sulfate) to unfold proteins for solid-state nanopore translocation, while simultaneously endowing them with a stronger electrical charge. Our simulations and experiments probe that SDS-treated proteins show considerable loss of protein structure during the nanopore translocation. Moreover, SDS-treated proteins translocate through the nanopore in the direction prescribed by the electrophoretic force fue to the negative charge impaired by SDS. In summary, our results suggest that SDS causes protein unfolding while facilitating protein translocation in the direction of the electrophoretic force, both characteristics could bring great advantages for future protein sequencing applications using solid-state nanopores.

Back to tab navigation

Supplementary files

Publication details

The article was received on 06 Apr 2017, accepted on 08 Jul 2017 and first published on 12 Jul 2017


Article type: Paper
DOI: 10.1039/C7NR02450A
Citation: Nanoscale, 2017, Accepted Manuscript
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    SDS-Assisted Protein Transport Through Solid-State Nanopores

    L. Restrepo-Pérez, S. John, A. Aksimentiev, C. Joo and C. Dekker, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR02450A

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements