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Simultaneous enzymatic activity modulation and rapid determination of enzyme kinetics by highly crystalline graphite dots

Abstract

The research field in enzyme-based biotechnology urgently requests discovery of new materials and methods with high-performance. Here we report that highly crystalline graphite dots (GDs) can modulate enzyme activities, and simultaneously allow for real-time measurements on enzyme kinetics in a combination with mass spectrometry (MS). A well-defined modulation of lipolytic activities from inhibition to enhancement can be realized by selectively coupling lipase enzymes with GDs containing specific functional groups on the surface. As a unique feature of our approach, GDs in the enzyme reaction can simultaneously serve as a versatile matrix for rapid and sensitive detection of the residual enzyme substrate, the intermediate or final product of lipolytic digestion using MS technology. Therefore, enzyme kinetic data can be collected in a real-time, high-throughput format. This work provides a new platform for enzymological research in hybrid bio-catalytic processes with the advanced nanotechnology.

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Publication details

The article was received on 31 Mar 2017, accepted on 16 May 2017 and first published on 18 May 2017


Article type: Paper
DOI: 10.1039/C7NR02285A
Citation: Nanoscale, 2017, Accepted Manuscript
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    Simultaneous enzymatic activity modulation and rapid determination of enzyme kinetics by highly crystalline graphite dots

    R. Shi, H. Li, E. Wu, L. Xiong, R. Lv, R. Guo, Y. Liu, G. Xu, Z. Kang and J. Liu, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR02285A

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