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Functional Insights into the Cellular Response Triggered by Bile-Acid Platinum Compound Conjugated to Biocompatible Ferric Nanoparticles Using Quantitative Proteomic Approaches

Abstract

At present, bioferrofluids are employed as multifunctional powerful tools for biomedical applications such as drug delivery, among others. The present study explores the cellular response when bile-acid platinum derivatives are conjugated with bioferrofluids by testing the biological activity in osteosarcoma (MG-63) and T-cell leukemia (Jurkat) cells. The aim of this work is to evaluate the biocompatibility of a bile-acid platinum derivative conjugated with multi-functional polymer coated bioferrofluids by observing the effects on the protein expression profiles and in intracellular pathways of the nanoparticle-stimulated cells. To this end, a mass spectrometry-based approach termed SILAC has been applied to determine in a high-throughput manner the key proteins involved in the cellular respond process (including specific quantitatively identified proteins related to the vesicular transport, cellular structure, cell cycle, biosynthetic process, apoptosis and regulation of the cell cycle). Finally, the biocompatibility was evaluated and validated also by conventional strategies (such as flow cytometry and MTT).

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Publication details

The article was received on 28 Mar 2017, accepted on 31 May 2017 and first published on 05 Jun 2017


Article type: Paper
DOI: 10.1039/C7NR02196H
Citation: Nanoscale, 2017, Accepted Manuscript
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    Functional Insights into the Cellular Response Triggered by Bile-Acid Platinum Compound Conjugated to Biocompatible Ferric Nanoparticles Using Quantitative Proteomic Approaches

    P. Díez, M. González-Muñoz, M. González-González, R. M. Dégano, R. Jara-Acevedo, S. Sánchez-Paradinas, R. Piñol, J. L. Murillo, G. Lou, F. Palacio, M. J. Almendral, A. Millán, E. Rodríguez-Fernández, J. J. Criado, N. Ibarrola and M. Fuentes, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR02196H

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