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Dynamic cell entry pathway of respiratory syncytial virus revealed by tracking the quantum dots-labeled single virus

Abstract

Studying the cell entry pathway at single-particle level can provide detailed and quantitative information for the dynamic events involved in virus entry. Indeed, viral entry dynamics cannot be monitored by static staining methods used in cell biology, thus virus dynamic tracking could be useful in the development of effective antiviral strategies. Therefore, the aim of this work was to use quantum dots-based single-particle tracking approach to monitor cell entry behavior of respiratory syncytial virus (RSV) in living cells. The time-lapse fluorescence imaging and trajectories analysis of the quantum dots-labeled RSV showed that RSV entry into HEp-2 cells consisted of a typical endocytosis trafficking process. Three critical events during RSV entry were observed according to entry dynamic and fluorescence colocalization analysis. Firstly, RSV was attached to lipid rafts of the cell membrane, then it was efficiently delivered into the perinuclear region within 2 h post-infection, mostly moving and residing into the lysosome compartment. Moreover, the relatively slow velocity of RSV transport across the cytoplasm and formation of actin tail indicated actin-based RSV motility, which also confirmed by the effects of cytoskeletal inhibitors. Taken together, these findings provided new insights into RSV entry mechanism and virus-cell interactions in RSV infection that could be beneficial in the development of antiviral drugs and vaccine.

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Supplementary files

Publication details

The article was received on 27 Mar 2017, accepted on 05 May 2017 and first published on 11 May 2017


Article type: Paper
DOI: 10.1039/C7NR02162C
Citation: Nanoscale, 2017, Accepted Manuscript
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    Dynamic cell entry pathway of respiratory syncytial virus revealed by tracking the quantum dots-labeled single virus

    L. L. Zheng, C. M. Li, S. J. Zhen, Y. F. Li and C. Z. Huang, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR02162C

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