Jump to main content
Jump to site search

Issue 27, 2017
Previous Article Next Article

Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles

Author affiliations

Abstract

The technological and economic benefits of engineered nanomaterials may be offset by their adverse effects on living organisms. One of the highly produced nanomaterials under such scrutiny is amorphous silica nanoparticles, which are known to have an appreciable, although reversible, inflammatory potential. This is due to their selective toxicity toward macrophages, and it is thus important to study the cellular responses of this cell type to silica nanoparticles to better understand the direct or indirect adverse effects of nanosilica. We have here studied the responses of the RAW264.7 murine macrophage cells and of the control MPC11 plasma cells to subtoxic concentrations of nanosilica, using a combination of proteomic and targeted approaches. This allowed us to document alterations in the cellular cytoskeleton, in the phagocytic capacity of the cells as well as their ability to respond to bacterial stimuli. More surprisingly, silica nanoparticles also induce a greater sensitivity of macrophages to DNA alkylating agents, such as styrene oxide, even at doses which do not induce any appreciable cell death.

Graphical abstract: Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles

Back to tab navigation

Supplementary files

Publication details

The article was received on 26 Mar 2017, accepted on 27 May 2017 and first published on 31 May 2017


Article type: Paper
DOI: 10.1039/C7NR02140B
Citation: Nanoscale, 2017,9, 9641-9658
  • Open access: Creative Commons BY-NC license
  •   Request permissions

    Differential proteomics highlights macrophage-specific responses to amorphous silica nanoparticles

    B. Dalzon, C. Aude-Garcia, V. Collin-Faure, H. Diemer, D. Béal, F. Dussert, D. Fenel, G. Schoehn, S. Cianférani, M. Carrière and T. Rabilloud, Nanoscale, 2017, 9, 9641
    DOI: 10.1039/C7NR02140B

    This article is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported Licence. Material from this article can be used in other publications provided that the correct acknowledgement is given with the reproduced material and it is not used for commercial purposes.

    Reproduced material should be attributed as follows:

    • For reproduction of material from NJC:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the Centre National de la Recherche Scientifique (CNRS) and the RSC.
    • For reproduction of material from PCCP:
      [Original citation] - Published by the PCCP Owner Societies.
    • For reproduction of material from PPS:
      [Original citation] - Published by The Royal Society of Chemistry (RSC) on behalf of the European Society for Photobiology, the European Photochemistry Association, and RSC.
    • For reproduction of material from all other RSC journals:
      [Original citation] - Published by The Royal Society of Chemistry.

    Information about reproducing material from RSC articles with different licences is available on our Permission Requests page.

Search articles by author

Spotlight

Advertisements