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An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

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Abstract

An efficient enzyme-powered micromotor device was fabricated by assembling multiple layers of catalase on the inner surface of a poly(3,4-ethylenedioxythiophene and sodium 4-styrenesulfonate)/Au microtube (PEDOT-PSS/Au). The catalase assembly was achieved by programmed DNA hybridization, which was performed by immobilizing a designed sandwich DNA structure as the sensing unit on the PEDOT-PSS/Au, and then alternately hybridizing with two assisting DNA to bind the enzyme for efficient motor motion. The micromotor device showed unique features of good reproducibility, stability and motion performance. Under optimal conditions, it showed a speed of 420 μm s−1 in 2% H2O2 and even 51 μm s−1 in 0.25% H2O2. In the presence of target DNA, the sensing unit hybridized with target DNA to release the multi-layer DNA as well as the multi-catalase, resulting in a decrease of the motion speed. By using the speed as a signal, the micromotor device could detect DNA from 10 nM to 1 μM. The proposed micromotor device along with the cyclic alternate DNA hybridization assembly technique provided a new path to fabricate efficient and versatile micromotors, which would be an exceptional tool for rapid and simple detection of biomolecules.

Graphical abstract: An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

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Publication details

The article was received on 16 Feb 2017, accepted on 28 May 2017 and first published on 02 Jun 2017


Article type: Paper
DOI: 10.1039/C7NR01168G
Citation: Nanoscale, 2017, Advance Article
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    An efficient enzyme-powered micromotor device fabricated by cyclic alternate hybridization assembly for DNA detection

    S. Fu, X. Zhang, Y. Xie, J. Wu and H. Ju, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR01168G

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