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Interaction of silver nanoparticles with metallothionein and ceruloplasmin: impact on metal substitution by Ag(I), corona formation and enzymatic activity

Abstract

The release of Ag(I) from silver nanoparticles (AgNPs) unintentionally spread in the environment is suspected to impair some key biological functions. In comparison with AgNO3, in-depth investigations were carried out into the interactions between citrate-coated AgNPs (20 nm) and two metalloproteins, intracellular metallothionein 1 (MT1) and plasmatic ceruloplasmin (Cp), both involved in metal homeostasis. These were chosen for their physiological relevance and the diversity of their various native metals bound because of thiol groups and/or their structural differences. Transmission electron microscopy (TEM), dynamic light scattering (DLS), UV-vis and circular dichroism (CD) spectroscopies were used to follow the effects of such intricate interactions on AgNP dissolution and proteins in terms of metal exchanges and structural modifications. The isolation of the different populations formed together with on-line quantifications of their metal content were performed by asymmetrical flow field-Flow fractionation (AF4) linked to inductively coupled plasma mass spectrometry (ICP-MS). For the 2 proteins, Ag(I) dissolved from the AgNPs, substituted for the native metal, to different extents and with different types of dynamics for the corona formed: the MT1 rapidly surrounded the AgNPs with transient reticulate corona thus promoting their dissolution associated with the metal substitution, whereas the Cp established a more stable layer around the AgNPs, with a limited substitution of Cu and a decrease in its ferroxidase activity. The accessibility and lability of the metal binding sites inside these proteins and their relative affinities for Ag(I) are discussed, taking into account the structural characteristics of the proteins.

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Publication details

The article was received on 14 Feb 2017, accepted on 12 Apr 2017 and first published on 13 Apr 2017


Article type: Paper
DOI: 10.1039/C7NR01075C
Citation: Nanoscale, 2017, Accepted Manuscript
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    Interaction of silver nanoparticles with metallothionein and ceruloplasmin: impact on metal substitution by Ag(I), corona formation and enzymatic activity

    W. Liu, I. A. M. Worms, N. Herlin, D. Truffier-Boutry, I. Michaud-Soret, E. Mintz, C. Vidaud and F. Rollin-Genetet, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C7NR01075C

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