Jump to main content
Jump to site search


A four-helix bundle DNA nanostructure with binding pockets for pyrimidine nucleotides

Author affiliations

Abstract

Designed DNA nanostructures of impressive size have been described, but designed structures of the size of protein enzymes that bind organic ligands with high specificity are rare. Here we report a four-helix motif consisting of three synthetic strands with 65 base pairs and 165 nucleotides in total that folds well. Furthermore, we show that in the interior of this small folded DNA nanostructure, cavities can be set up that bind pyrimidine nucleotides with micromolar affinity. Base-specific binding for both thymidine and cytidine derivatives is demonstrated. The binding affinity depends on the position in the structure, as expected for recognition beyond simple base pairing. The folding motif reported here can help to expand DNA nanotechnology into the realm of selective molecular recognition that is currently dominated by protein-based enzymes and receptors.

Graphical abstract: A four-helix bundle DNA nanostructure with binding pockets for pyrimidine nucleotides

Back to tab navigation
Please wait while Download options loads

Supplementary files

Publication details

The article was received on 04 Jan 2017, accepted on 06 Mar 2017 and first published on 09 Mar 2017


Article type: Paper
DOI: 10.1039/C7NR00094D
Citation: Nanoscale, 2017, Advance Article
  •   Request permissions

    A four-helix bundle DNA nanostructure with binding pockets for pyrimidine nucleotides

    R. J. Schwarz and C. Richert, Nanoscale, 2017, Advance Article , DOI: 10.1039/C7NR00094D

Search articles by author