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Issue 17, 2017
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Mapping cell behavior across a wide range of vertical silicon nanocolumn densities

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Abstract

Over the past decade, vertical nanostructures have provided novel approaches for biomedical applications such as intracellular delivery/detection, specific cell capture, membrane potential measurement, and cellular activity regulation. Although the feasibility of the vertical nanostructures as a new biological tool has been thoroughly demonstrated, a better understanding of cell behavior on vertical nanostructures, in particular the effects of geometry, is essential for advanced applications. To investigate the cell behavior according to the variation of the spacing between vertical nanostructures, we have interfaced fibroblasts (NIH3T3) with density-controlled vertical silicon nanocolumn arrays (vSNAs). Over a wide range of vSNA densities, we observe three distinct cell settling regimes and investigate both overall cell behavior (adhesions, morphology, and mobility) and detailed biomacromolecule variance (F-actin and focal adhesion) across these regimes. We expect that these systematic observations could serve as a guide for improved nanostructure array design for the desired cell manipulation.

Graphical abstract: Mapping cell behavior across a wide range of vertical silicon nanocolumn densities

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Publication details

The article was received on 16 Dec 2016, accepted on 29 Mar 2017 and first published on 04 Apr 2017


Article type: Paper
DOI: 10.1039/C6NR09700F
Citation: Nanoscale, 2017,9, 5517-5527
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    Mapping cell behavior across a wide range of vertical silicon nanocolumn densities

    N. Buch-Månson, D. Kang, D. Kim, K. E. Lee, M. Yoon and K. L. Martinez, Nanoscale, 2017, 9, 5517
    DOI: 10.1039/C6NR09700F

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