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Nanoparticle-induced oxidation of corona proteins initiates an oxidative stress response in cells

Abstract

Titanium dioxide nanoparticles (TiO2 NPs), used as pigments and photocatalysts, are ubiquitous in our daily lives. Previous work has observed cellular oxidative stress in response to UV-excitation of photocatalytic TiO2 NPs. In comparison, most human exposure to TiO2 NPs takes place in the dark; in the lung following inhalation or in the gut following consumption of TiO2 NP food pigment. Our spectroscopic characterization shows that both photocatalytic and food grade TiO2 NPs, in the dark, generate low levels of reactive oxygen species (ROS), specifically hydroxyl radicals and superoxide. These ROS oxidize serum proteins that form a corona of proteins on the NP surface. This protein layer is the interface between the NP and the cell. An oxidized protein corona triggers an oxidative stress response, detected with PCR and western blot. Surface modification of TiO2 NPs to increase or decrease surface defects correlates with ROS generation and oxidative stress, suggesting NP surface defects, especially oxygen vacancies, are the underlying cause of TiO2 NP-induced oxidative stress.

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Publication details

The article was received on 08 Dec 2016, accepted on 28 Apr 2017 and first published on 03 May 2017


Article type: Paper
DOI: 10.1039/C6NR09500C
Citation: Nanoscale, 2017, Accepted Manuscript
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    Nanoparticle-induced oxidation of corona proteins initiates an oxidative stress response in cells

    D. T. Jayaram, S. Runa, M. L. Kemp and C. Payne, Nanoscale, 2017, Accepted Manuscript , DOI: 10.1039/C6NR09500C

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