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Issue 12, 2017
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Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

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Abstract

We report the temperature dependence of metal-enhanced fluorescence (MEF) of individual photosystem I (PSI) complexes from Thermosynechococcus elongatus (T. elongatus) coupled to gold nanoparticles (AuNPs). A strong temperature dependence of shape and intensity of the emission spectra is observed when PSI is coupled to AuNPs. For each temperature, the enhancement factor (EF) is calculated by comparing the intensity of individual AuNP-coupled PSI to the mean intensity of ‘uncoupled’ PSI. At cryogenic temperature (1.6 K) the average EF was 4.3-fold. Upon increasing the temperature to 250 K the EF increases to 84-fold. Single complexes show even higher EFs up to 441.0-fold. At increasing temperatures the different spectral pools of PSI from T. elongatus become distinguishable. These pools are affected differently by the plasmonic interactions and show different enhancements. The remarkable increase of the EFs is explained by a rate model including the temperature dependence of the fluorescence yield of PSI and the spectral overlap between absorption and emission spectra of AuNPs and PSI, respectively.

Graphical abstract: Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

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Publication details

The article was received on 09 Nov 2016, accepted on 21 Feb 2017 and first published on 23 Feb 2017


Article type: Paper
DOI: 10.1039/C6NR08762K
Citation: Nanoscale, 2017,9, 4196-4204
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    Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

    I. Ashraf, A. Konrad, H. Lokstein, S. Skandary, M. Metzger, J. M. Djouda, T. Maurer, P. M. Adam, A. J. Meixner and M. Brecht, Nanoscale, 2017, 9, 4196
    DOI: 10.1039/C6NR08762K

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