Issue 12, 2017

Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

Abstract

We report the temperature dependence of metal-enhanced fluorescence (MEF) of individual photosystem I (PSI) complexes from Thermosynechococcus elongatus (T. elongatus) coupled to gold nanoparticles (AuNPs). A strong temperature dependence of shape and intensity of the emission spectra is observed when PSI is coupled to AuNPs. For each temperature, the enhancement factor (EF) is calculated by comparing the intensity of individual AuNP-coupled PSI to the mean intensity of ‘uncoupled’ PSI. At cryogenic temperature (1.6 K) the average EF was 4.3-fold. Upon increasing the temperature to 250 K the EF increases to 84-fold. Single complexes show even higher EFs up to 441.0-fold. At increasing temperatures the different spectral pools of PSI from T. elongatus become distinguishable. These pools are affected differently by the plasmonic interactions and show different enhancements. The remarkable increase of the EFs is explained by a rate model including the temperature dependence of the fluorescence yield of PSI and the spectral overlap between absorption and emission spectra of AuNPs and PSI, respectively.

Graphical abstract: Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

Article information

Article type
Paper
Submitted
09 Nov 2016
Accepted
21 Feb 2017
First published
23 Feb 2017

Nanoscale, 2017,9, 4196-4204

Temperature dependence of metal-enhanced fluorescence of photosystem I from Thermosynechococcus elongatus

I. Ashraf, A. Konrad, H. Lokstein, S. Skandary, M. Metzger, J. M. Djouda, T. Maurer, P. M. Adam, A. J. Meixner and M. Brecht, Nanoscale, 2017, 9, 4196 DOI: 10.1039/C6NR08762K

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