Jump to main content
Jump to site search

Issue 1, 2017
Previous Article Next Article

Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

Author affiliations

Abstract

Covering: 2000 to 2016

The labor-intensive process of microbial natural product discovery is contingent upon identifying discrete secondary metabolites of interest within complex biological extracts, which contain inventories of all extractable small molecules produced by an organism or consortium. Historically, compound isolation prioritization has been driven by observed biological activity and/or relative metabolite abundance and followed by dereplication via accurate mass analysis. Decades of discovery using variants of these methods has generated the natural pharmacopeia but also contributes to recent high rediscovery rates. However, genomic sequencing reveals substantial untapped potential in previously mined organisms, and can provide useful prescience of potentially new secondary metabolites that ultimately enables isolation. Recently, advances in comparative metabolomics analyses have been coupled to secondary metabolic predictions to accelerate bioactivity and abundance-independent discovery work flows. In this review we will discuss the various analytical and computational techniques that enable MS-based metabolomic applications to natural product discovery and discuss the future prospects for comparative metabolomics in natural product discovery.

Graphical abstract: Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

Back to tab navigation

Publication details

The article was received on 22 Apr 2016 and first published on 07 Sep 2016


Article type: Review Article
DOI: 10.1039/C6NP00048G
Citation: Nat. Prod. Rep., 2017,34, 6-24
  •   Request permissions

    Comparative mass spectrometry-based metabolomics strategies for the investigation of microbial secondary metabolites

    B. C. Covington, J. A. McLean and B. O. Bachmann, Nat. Prod. Rep., 2017, 34, 6
    DOI: 10.1039/C6NP00048G

Search articles by author

Spotlight

Advertisements