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FRET based selective and ratiometric detection of Al(III) with live-cell imaging

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Abstract

A new FRET-based carbazole rhodamine hybrid (CRH) ratiometric fluorescent sensor is reported for the selective detection of Al3+. The design of the probe involved two parts: a carbazole hydrazone energy donor and a rhodamine spirolactone unit (potential rhodamine monoanion energy receptor). First, the emission of the carbazole chromophore (“donor” moiety) and next the absorption of the ring-opened rhodamine dye were measured, which clearly showed the overlap between these two spectra, indicating the FRET from the carbazole chromophore to the rhodamine moiety. FRET based ratiometric live-cell imaging for Al3+ has been rarely reported. Successful live cell imaging is demonstrated, where on addition of Al3+ solution (20 μL), the color of the CRH (20 μL) treated cells changed from blue to red.

Graphical abstract: FRET based selective and ratiometric detection of Al(iii) with live-cell imaging

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Publication details

The article was received on 18 Aug 2017, accepted on 17 Oct 2017 and first published on 18 Oct 2017


Article type: Paper
DOI: 10.1039/C7NJ03079G
Citation: New J. Chem., 2017, Advance Article
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    FRET based selective and ratiometric detection of Al(III) with live-cell imaging

    A. Manna, D. Sain, N. Guchhait and S. Goswami, New J. Chem., 2017, Advance Article , DOI: 10.1039/C7NJ03079G

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