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Influence of cholinium-based ionic liquids on the structural stability and activity of α-chymotrypsin

Abstract

In recent years, the potential of α-chymotypsin (CT) as biocatalysts has mushroomed new areas of its application ranging from pharmaceutical to chemicals industries. However, high thermal stability is one of the major challenges to the use of this enzyme in biocatalysis. In this regards, ionic liquids (ILs) have been used as promising media for the stabilization and preservation of proteins, enzymes, DNA and other biomolecules. In present study, it was found that a series of cholinium-based ILs such as choline acetate ([Ch][Ac]), choline chloride ([Ch][Cl]), and choline dihydrogen phosphate ([Ch][Dhp]) stabilised the CT structure against thermal denaturation. The transition temperature (Tm) of CT was increased from ~48.9 °C (in buffer) to 58 °C (in ILs media). Enzymatic activity of CT in the presence of ILs was also monitored by using casein as the substrate. It was found that choline dihydrogen citrate ([Ch][Dhc]) and choline hydroxide ([Ch][OH]) dramatically decrease the enzyme activity. While both structural stability and enzymatic activity was retained in [Ch][Ac], [Ch][Cl] and [Ch][Dhp] indicating the suitability of these ILs as a high temperature bio-catalytic reactor. Our results revealed that [Ch][Ac] is best stabilizer among all studied ILs for the native structure of CT whereas [Ch][OH] is strongest destabilizer for CT structure. The outcome of our results can be helpful to overcome some of the major limitations found in the development of biocatalysis processes

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Publication details

The article was received on 14 Aug 2017, accepted on 12 Oct 2017 and first published on 12 Oct 2017


Article type: Paper
DOI: 10.1039/C7NJ03023A
Citation: New J. Chem., 2017, Accepted Manuscript
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    Influence of cholinium-based ionic liquids on the structural stability and activity of α-chymotrypsin

    M. Bisht and P. Venkatesu, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ03023A

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