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Green Biosynthesis of CuO & Ag-CuO nanoparticles from Malus Domestica leaf extract and evaluation of antibacterial, antioxidant, DNA cleavage activities

Abstract

The reactivity of metallic and bimetallic nanoparticles of copper and silver towards In vitro study has been quantitatively investigated. Here, we report an easy synthesis of nanoparticles by the green method using Malus domestica leaf extract which acts as a good stabilizing agent. The synthesized CuO and Ag-CuO nanoparticles have shown Surface Plasmon Resonance maxima (SPR) at 335 and 440 nm respectively. The particles are of spherical and crystalline in nature with average sizes between 18 - 20 nm. The Malus domestica leaves capped nanoparticles have exhibited interesting antibacterial activity with both gram-positive and gram-negative bacteria at microgram concentrations. The antioxidant activity of the synthesized nanoparticles has also been measured on the basis of the free radical scavenging activity by the DPPH (1, 1- diphenyl 2-picrylhydrazyl) method. Moreover, the DNA cleavage activities of the synthesized nanoparticles have been screened by agarose gel electrophoresis using E. coli pBR322 plasmid as a target. Hence owing to the biological active nature of the synthesized nanoparticles, these green synthesized NPs act as potent therapeutic agent.

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Publication details

The article was received on 13 Aug 2017, accepted on 06 Nov 2017 and first published on 08 Nov 2017


Article type: Paper
DOI: 10.1039/C7NJ02977B
Citation: New J. Chem., 2017, Accepted Manuscript
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    Green Biosynthesis of CuO & Ag-CuO nanoparticles from Malus Domestica leaf extract and evaluation of antibacterial, antioxidant, DNA cleavage activities

    M. Jadhav, S. Kulkarni, P. Raikar, D. A. Barretto, S. K. Vootla and U. S. Raikar, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ02977B

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