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Polypyrrole-methylanthranilate functionalized “worm-like” titanium dioxide nanocomposite as an innovative tool for immobilization of lipase: Preparation, activity-stability and molecular docking investigations

Abstract

Enzymatic biocatalysis holds vast reputation on an industrial scale. The major hindrance faced by enzymes is of denaturation under various conditions. In this work, we report that activity and stability of the enzymes can be enhanced by their immobilization on novel synthesized amine groups containing polypyrrole-methylanthranilate titanium oxide nanocomposite. We show this by successfully immobilizing lipase from Rhizopus oryzae on Ppy-MA/TiO2 NC by physical adsorption and glutaraldehyde activated covalent coupling procedure. The catalytic efficiency of the free and immobilized preparation was determined for the hydrolysis of p-nitrophenyl palmitate. The covalently immobilized lipase showed significantly higher activity yield (effectiveness factor was 0.97) in comparison with the adsorbed counterpart (effectiveness factor of 0.86). The binding of lipase to Ppy-MA/TiO2-NC was affirmed by transmission electron microscopy, fourier transform infrared spectroscopy, scanning electron microscopy and dynamic light scattering. TGA and DTA were performed to inspect the thermal stability of the synthesised bioconjugates. The storage stability, solvent tolerance, the reusability of the resulting nano-biocatalyst and the effect of pH and temperature on the catalytic activities were also evaluated. The prepared nanobioconjugates displayed remarkable improved against solvent tolerance (150% and 125% activity recovery in acetone and isopropanol) as compared to its free counterpart. We envisage that covalent binding method played a profound role in refining the properties of the enzyme immobilized on Ppy-MA/TiO2 NC. In view of the above, the hydrolytic enzyme immobilized on this functionalized TiO2 nanocarriers can be used as a stable and effective nano-biocatalyst with very vital implications in industrial biotechnology.

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Publication details

The article was received on 10 Aug 2017, accepted on 12 Nov 2017 and first published on 13 Nov 2017


Article type: Paper
DOI: 10.1039/C7NJ02951A
Citation: New J. Chem., 2017, Accepted Manuscript
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    Polypyrrole-methylanthranilate functionalized “worm-like” titanium dioxide nanocomposite as an innovative tool for immobilization of lipase: Preparation, activity-stability and molecular docking investigations

    S. Asmat, Q. Husain and M. S. Khan, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ02951A

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