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A convenient and label-free colorimetric assay for dopamine detection based on the inhibition of Cu (II)-catalyzed oxidation of 3, 3´, 5, 5´-tetramethylbenzidine-H2O2 system

Abstract

In this study, a simple and label-free colorimetric sensing strategy was reported for rapid and selective detection of dopamine by inhibiting Cu2+-catalyzed oxidation of 3, 3´, 5, 5´-tetramethylbenzidine (TMB)-H2O2 reaction. Similar to natural peroxidase, Cu2+ could catalyze the oxidation of the peroxidase substrate TMB to oxidized TMB (ox TMB) in the presence of H2O2, producing a blue color. However, dopamine could chelate with Cu2+ to form stable dopamine-Cu2+ complexes by strong coordination between Cu2+ and dopamine. It was found that the formation of dopamine-Cu2+ complexes hindered the Cu2+-catalyzed oxidation of TMB-H2O2 reaction. As a result, in the presence of dopamine, the solution color changed from blue to colorless with a remarkable decrease of absorbance. Under the optimized experimental conditions, the colorimetric sensor exhibited linear range of 1 μM to 50 μM for dopamine detection, with detection limit of 1 μM. Furthermore, the proposed method was successfully applied to determinate the content of dopamine in real samples. Compared with other reported methods, the colorimetric method performed within several minutes and did not require any complex or time-consuming preparation and purification process. The results suggested that this strategy would be expected to have potential applications in biotechnology and clinical diagnosis.

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Publication details

The article was received on 25 Jul 2017, accepted on 10 Oct 2017 and first published on 11 Oct 2017


Article type: Paper
DOI: 10.1039/C7NJ02710A
Citation: New J. Chem., 2017, Accepted Manuscript
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    A convenient and label-free colorimetric assay for dopamine detection based on the inhibition of Cu (II)-catalyzed oxidation of 3, 3´, 5, 5´-tetramethylbenzidine-H2O2 system

    H. Wang, Y. Li, G. Dong, T. Gan and Y. Liu, New J. Chem., 2017, Accepted Manuscript , DOI: 10.1039/C7NJ02710A

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